Journal:

Article Title: B-cell Receptor Activation Induces BIC/miR-155 Expression through a Conserved AP-1 Element *

doi: 10.1074/jbc.M708218200

Figure Lengend Snippet: Ramos cells were pretreated with the indicated inhibitors prior to the addition of anti-IgM. Cells were lysed and subjected to Western blot analysis as described under “Experimental Procedures.” MEK1/2 Inh., MEK1/2 inhibition; JNK Inh., JNK inhibition; p38 Inh., p38 inhibition; DMSO, Me2SO; p-ERK, phospho-ERK.

Article Snippet: Ramos cells were cultured for 2 h in the absence or presence of 10 μ m anti-IgM (Sigma, catalog number I 0759) (1 × 10 7 cells were used for each immunoprecipitation).

Techniques: Western Blot, Inhibition

Journal:

Article Title: B-cell Receptor Activation Induces BIC/miR-155 Expression through a Conserved AP-1 Element *

doi: 10.1074/jbc.M708218200

Figure Lengend Snippet: A and B, electrophoretic mobility shift analysis was carried out using the BIC AP-1 promoter element and extracts from uninduced and anti-IgM induced Ramos cells. Competitor oligonucleotides sequences and antibodies used are described under “Experimental Procedures.” control Ab, control antibody. C, Ramos cells were transfected with either the wild type BIC reporter (wt) or the AP-1 site mutant BIC reporter (mAP-1) along either with 1 μg of control, JunB, or FosB expression vectors or with 0.5 μg of JunB plus 0.5 μg of FosB expression vector. All values are relative to wild type promoter activity plus control expression vectors. CNTL, control.

Article Snippet: Ramos cells were cultured for 2 h in the absence or presence of 10 μ m anti-IgM (Sigma, catalog number I 0759) (1 × 10 7 cells were used for each immunoprecipitation).

Techniques: Electrophoretic Mobility Shift Assay, Transfection, Mutagenesis, Expressing, Plasmid Preparation, Activity Assay

Journal:

Article Title: B-cell Receptor Activation Induces BIC/miR-155 Expression through a Conserved AP-1 Element *

doi: 10.1074/jbc.M708218200

Figure Lengend Snippet: Ramos cells were pre-treated with the indicated inhibitors prior to the addition of anti-IgM. Total RNAs were then analyzed for BIC (A) and miR-155 (B) expression by real-time reverse transcription-PCR. All values are relative to G3PDH expression.

Article Snippet: Ramos cells were cultured for 2 h in the absence or presence of 10 μ m anti-IgM (Sigma, catalog number I 0759) (1 × 10 7 cells were used for each immunoprecipitation).

Techniques: Expressing

Journal: Journal of Physics D

Article Title: Surface plasmon resonance imaging of excitable cells

doi: 10.1088/1361-6463/aaf849

Figure Lengend Snippet: The sensitivity of the SPR imaging system. The refractive index was adjusted using a series of different concentration NaCl solutions. A 1 mW 680 nm SLED light source was used with a 12-bit CMOS camera and a 3.5 ms exposure time. The sensitivity in RIU was calculated as 2 × 10 −5 . The linear fit is \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$y=({{p}_{1}}\times {\rm RI})+{{p}_{2}}$ \end{document} y = ( p 1 × R I ) + p 2 , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}${{p}_{1}}$ \end{document} p 1 = 39.137 and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}${{p}_{2}}$ \end{document} p 2 = −51.72. R 2 = 0.9913.

Article Snippet: The BFP was monitored on a complementaty metal oxide semiconductor (CMOS) camera (Unibrain, 640 × 480 pixels, 5.6 μ m pixel size, Fire-i TM , Unibrain Inc., San Ramon, CA, USA), shown in figure .

Techniques: Imaging, Refractive Index, Concentration Assay

Journal:

Article Title: Sensitivity of an Epstein-Barr Virus-Positive Tumor Line, Daudi, to Alpha Interferon Correlates with Expression of a GC-Rich Viral Transcript

doi:

Figure Lengend Snippet: Expression levels of EBV D-HIT and homologs in B-cell lines

Article Snippet: Since this transcript might represent an endogenous double-stranded modulator of interferon pathways, we studied its potential for explaining the differential sensitivity to IFN-α among Daudi isolates and other EBV-carrying cells. table ft1 table-wrap mode="anchored" t5 caption a7 Cells Transcription a Uninduced Induced b Daudi/ATCC + +++ Daudi/ICRF ++ +++++ M-ABA − + Namalwa − − NAD-C15 − − P3HR1 ++ ++ Raji − + Ramos − − Open in a separate window a + to +++++ indicate levels of transcription relative to those observed by Northern blot analyses under comparable conditions.

Techniques: Expressing

Journal:

Article Title: Sensitivity of an Epstein-Barr Virus-Positive Tumor Line, Daudi, to Alpha Interferon Correlates with Expression of a GC-Rich Viral Transcript

doi:

Figure Lengend Snippet: Interferon sensitivities of Daudi/ATCC cells. Cells were grown in the presence of various concentrations of IFN-α over a 28-day period, and the number of live cells (as a percentage of untreated cells) was plotted. The profile of Daudi/ATCC, showing viable cell counts as a percentage of cells grown in the absence of interferon (100%), is taken as a standard for medium IFN-α sensitivity. Superimposed on it are two curves from Daudi/ICRF cells (grown in the presence of 1 and 10 U of IFN-α/ml; high sensitivity), one from Raji cells (grown with 104 U; low sensitivity), and one from P3HR1 cells (102 U; high/medium sensitivity; data taken from the literature [2]), all EBV-positive lines.

Article Snippet: Since this transcript might represent an endogenous double-stranded modulator of interferon pathways, we studied its potential for explaining the differential sensitivity to IFN-α among Daudi isolates and other EBV-carrying cells. table ft1 table-wrap mode="anchored" t5 caption a7 Cells Transcription a Uninduced Induced b Daudi/ATCC + +++ Daudi/ICRF ++ +++++ M-ABA − + Namalwa − − NAD-C15 − − P3HR1 ++ ++ Raji − + Ramos − − Open in a separate window a + to +++++ indicate levels of transcription relative to those observed by Northern blot analyses under comparable conditions.

Techniques: